資訊中心 > 產品文獻集 > Microarray Technology (15)

  ✔本篇論文使用華聯產品:Array technology and applications  
 Chemical Physics Letters. 2004, 397: 429¡V434. doi:10.1117/12.647449.
 An investigation into the influence of secondary structures on DNA hybridization using surface plasmon resonance biosensing 
 Fan-ching Chien, Jih-shiou Liu, Huang-ju Su, Li-an Kao, Chung-fan Chiou, Wen-yih Chen, Shean-jen Chen
  Abstract
This study utilizes a surface plasmon resonance (SPR) biosensing and a theoretical secondary structure calculation to investigate the influence of secondary structures on the DNA hybridization. It is found that the SPR angular shifts associated with the three pairs of 60mer oligonucleotides with prominent secondary structures are lower than those observed for the two pairs of oligonucleotides with no obvious secondary structures. It is also determined that increasing the DNA hybridization temperature from 35 to 45 ¢XC reduces secondary structure effects. On the hybridization with mixture target oligonucleotides, the SPR results demonstrate that secondary structures interfere significantly.
   

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 Journal Of Laboratory Automation. 2012, 17(6):405-407. doi: 10.1177/2211068212463689.
 Lab Automation Services
 
 
 Stephen Hughes
  Abstract

   

  ✔本篇論文使用華聯產品:Array technology and applications  
 Neurology Today. 2011, 11(19): 24-27. doi: 10.1097/01.NT.0000407216.70062.aa.
 Evidence Report: Genetic, Metabolic Screening Useful in children with Intellectual Development Deficits
 
 
 Kurt Samson
  Abstract
Although there are challenges yet to be overcome in the diagnostic evaluation of children with unexplained global developmental delay or intellectual disability (GDD/ID), genetic and metabolic screening tests are increasingly useful, according to a review by the AAN Quality Standards Subcommittee and the Practice Committee of the Child Neurology Society. The review was published in the Sept. 28 online edition of Neurology. In an interview with Neurology Today, AAN subcommittee panelist David J. Michelson, MD, assistant professor of neurology and pediatrics at the Loma Linda University School of Medicine in California, discussed the fi ndings and their implications for clinicians.
   

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 Pediatrics & Neonatology. 2014 Nov 4. doi:10.1016/j.pedneo.2014.08.001.
 Genetic Evaluation of Children with Global Developmental Delay¡XCurrent Status of Network Systems in Taiwan
 
 
 Shio-jean Lin, Yong-lin Foo, Julie Chi Chow, Ming-chi Lai, Wen-hui Tsai, Li-chen Tung, Mei-chin Kuo
  Abstract
This review article aims to introduce the screening and referral network of genetic evaluation for children with developmental delay in Taiwan. For these children, integrated systems provide services from the medical, educational, and social welfare sectors. All cities and counties in Taiwan have established a network for screening, detection, referral, evaluation, and intervention services. Increased awareness improves early detection and intervention. There remains a gap between supply and demand, especially with regard to financial resources and professional manpower. Genetic etiology has a major role in prenatal causes of developmental delay. A summary of reports on some related genetic disorders in the Taiwanese population is included in this review. Genetic diagnosis allows counseling with regard to recurrence risk and prevention. Networking with neonatal screening, laboratory diagnosis, genetic counseling, and orphan drugs logistics systems can provide effective treatment for patients. In Taiwan, several laboratories provide genetic tests for clinical diagnosis. Accessibility to advanced expensive tests such as gene chips or whole exome sequencing is limited because of funding problems; however, the service system in Taiwan can still operate in a relatively cost-effective manner. This experience in Taiwan may serve as a reference for other countries.
   

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 Advances In Clinical Chemistry. 2014 Nov 4. doi:10.1016/bs.acc.2014.09.007.
 Circulating microRNAs as Promising Tumor Biomarkers
 
 
 Qing H. Meng, Meng Chen, George A. Calin
  Abstract
microRNAs (miRNAs) are small, nonprotein-coding RNAs that function as posttranscriptional regulators of target genes. miRNAs are involved in multiple cell differentiation, proliferation, and apoptosis processes that are closely related to tumorigenesis. Circulating miRNAs are promising cancer biomarkers under development with great translational potential in personalized medicine. Here, we describe the origin and function of circulating miRNAs and compare the current new high-throughput technology applied to miRNA quantitation. The latest publications on circulating miRNAs were summarized, indicating that miRNAs are potential biomarkers of diagnosis, prognosis, and treatment response of major cancer types including prostate, breast, lung, colorectal, and hematological cancers. We addressed the strengths and limitations of applying circulating miRNAs in clinical laboratory and several issues associated with the accurate measurement of circulating miRNAs.