Human OneArray®
Over 30,000 Probes Give You
a Wealth of Information!
  Probe annotation search engine

Phalanx Annotation Search System
Product Description and Overview
30,275 total features
  • 29,187 human genome probes and 1,088 experimental control probes
  • Highly sensitive 60-mer sense-strand polynucleotide probes
  • 1" X 3" (25mm x 75mm) standard-format glass slide
  • Latest information of human genome content from public domains is used to design the probes that monitor the expression level of corresponding human protein-coding genes
Each probe is spotted onto the array in a highly consistent manner using an innovative non-contact spotting technology adapted and perfected by Phalanx Biotech for microarray manufacturing.

OneArray® Advantages
  • 100% satisfaction & replacement guarantee
     
  • Outstanding customer and technical support team
     
  • Competes head-to-head with popular platforms
  • Highly competitive pricing  << More>>
     
  • Specific 60-mer oligonucleotide probes
     
  • Uniform, non-contact printing technology

A
B
Figure 1: Post Hybridization of Human OneArray® using single colors, indirect labeling of aRNA. A) shows the full microarray; B) is a magnified region to show clarity and spot morphology.

Probe Set Content
  • Each 60-mer oligonucleotide probe is designed to hybridize to a specific target gene described in the latest public domain contents, such as NCBI Reference Sequence (RefSeq) Database and Ensembl Database, and validated by the Human Genome Sequencing Project.

  • The most well-known and studied target genes are the first priority of probe selection.

  • 1088 performance monitoring control probes for hybridization, sample quality, and labeling reactions.

  • Also included are the ArrayControl™ Sense Oligo Spots by Ambion.

  • Downloadable gene lists, probe sequences, and whole genome GAL files. << More>>
Human OneArray® Probe Set Content
Probe Type Number of Probes
Total Probes 30,275*
Probe Design based:
          - RefSeq release 38
          - Ensembl release 56		 29,187
Control Probes 1,088
*Human OneArray® is guaranteed to have > 98% of the total probe content printed.
Table 1 (above): Human OneArray® Probe Content

Human OneArray® Performance
Table 2 (right) displays results obtained using 5 Human OneArray® DNA Microarrays with 2.5 ug of total RNA from the Brain Reference RNA from Stratagene with one round of amplification using Amino Allyl MessageAmp™ Kit from Ambion.

For Hybridization, 10 ug of aRNA was labeled with Cyanine 5 fluorescent, and scanned with an Axon scanner*.
Human OneArray® Whole Genome Hybridization
  Spot Number Spot Percentage Average Signal Intensity
Average Value 24,926.0 85.4% 5,803.0
CV 2.0% 2.0% 3.8%
Table 2 (right): Human OneArray® Repeatability

To learn more about the OneArray® advantages, visit The Power of OneArray®. << More>>
*Axon scanner data:
PMT/Power: 600/100;
Filter: F635.Median - B636.Mean >2.6 B635 sd.

Control Features
  Visit our Experimental Controls support page to learn more about how to use the 1,088 experimental control probes on Human OneArray®. << More>>