Human OneArray™
Over 30,000 Probes Give You
a Wealth of Information!
Product Description and Overview
32,050 total features
  • 30,968 human genome probes, and 1082 experimental control probes
  • Highly sensitive 60-mer sense-strand polynucleotide probes
  • 1" X 3" (25mm x 75mm) standard-format glass slide
  • Latest information of human genome content from public domains is used to design the probes that monitor the expression level of corresponding human protein-coding genes
Each probe is spotted onto the array in a highly consistent manner using an innovative non-contact spotting technology adapted and perfected by Phalanx Biotech for microarray manufacturing.

OneArray Advantages
  • 100% satisfaction & replacement guarantee
     
  • Outstanding customer and technical support team
     
  • Competes head-to-head with popular platforms
  • Highly competitive pricing  << More>>
     
  • Specific 60-mer oligonucleotide probes
     
  • Uniform, non-contact printing technology

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B
Figure 1: Post Hybridization of Human OneArray using dual colors, indirect labeling of aRNA. A) shows the full microarray; B) is a magnified region to show clarity and spot morphology.

Probe Set Content
  • Each 60-mer oligonucleotide probe is designed to hybridize to a specific target gene described in the latest public domain contents, such as Unigene, Cancer Genome Anatomy Project (CGAP), BioCarta, Kyoto Encyclopedia of Gene and Genome (KEGG), and validated by the Human Genome Sequencing Project.

  • The most well-known and studied target genes are the first priority of probe selection.

  • 1082 performance monitoring control probes for hybridization, sample quality, and labeling reactions.

  • Also included are the ArrayControl™ Sense Oligo Spots by Ambion.

  • Downloadable gene lists, probe sequences, and whole genome GAL files. << More>>
Human OneArray™ Probe Set Content
Probe Type Number of Probes
UniGene and Refseq based 30,968 (total)*
UniGene build #175-based and/or Refseq based with Entrez Gene ID including:
CGAP (Cancer Genome Anatomy Project)
Biocarta and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways		 24,858
Unigene Build #163-based with Gene ID and experimentally selected 2265
*Human OneArray is guaranteed to have > 98% of the total probe content printed.
Table 1 (above): Human Genome Content

Human OneArray Performance
Table 2 (right) displays results obtained using 10 Human OneArray DNA Microarrays with 5 ug of total RNA from the Universal Reference RNA from Stratagene with one round of amplification using Amino Allyl MessageAmp™ Kit from Ambion.

For Hybridization, 10 ug of aRNA was labeled with Cyanine 5 fluorescent, and scanned with an Agilent scanner*.
Human OneArray™ Whole Genome Hybridization
  Spot Number Spot Percentage Average Signal Intensity
Average Value 27,425 88.6 1527.5
CV 1.5% 1.5% 7.4%
Table 2 (right): Human OneArray Repeatability

To learn more about the OneArray advantages, visit The Power of OneArray. << More>>
*Agilent scanner data:
PMT: 100/10;
Filter: F635.Median - B636.Mean >2.6 B635 sd.

Control Features
  Visit our Experimental Controls support page to learn more about how to use the 1,082 experimental control probes on Human OneArray. << More>>