Phalanx Human Whole Genome OneArray^TM     

Standard Microarray Format:

  Human OneArray^TM is printed on 1” x 3” (25mm x 75mm) standard

     glass slide with barcode

  Total of 32,050 oligonucleotide probes on a single microarray: 30,968

     human genome probes and 1,082 experimental control probes

  Each array contains necessary controls for customers to monitor

     performance

  Each human target gene is represented by highly specific 60mer

     oligonucleotide probe

Open System & Highly Compatible Platform:

  Human Whole Genome OneArray^TM is compatible with the majority of

     commercially available labeling reagents from Ambion, Clontech, Enzo

     Life Science, Invitrogen, Kreatech, Stratagene,  and others

  Human Whole Genome OneArray^TM can be used with Cyanine 3,

     Cyanine 5, and other compatible dyes

  Human Whole Genome OneArray^TM is manufactured in a format that

     is easily adapted with existing hybridization methods & systems,

     commercial scanners, analysis systems, and analysis software

     packages

Commercial Quality and Performance:

  Array to array reproducibility: average R² value of same sample

     comparison is higher than 0.95

  Dynamic range: ~2-3 orders of magnitude

  Detection sensitivity: ~0.05pM

User Support:

  100% satisfaction guarantee

   Probe annotation and scanner files

   User manual and supplementary protocols

   Easy access to knowledgeable technical support representatives

Bioinformatics Content:

Phalanx Human Whole Genome OneArray™ employs IMPORT (INTEGRATED MASSIVE PROBES’ OPTIMAL RECOGNITION TOOL) to select probes for gene transcript detection by hybridization.  The design and selection of the probe set are based on the following stringent parameters to deliver the highest specificity and hybridization efficiency:

  Lowest possible hexamer frequency score

  Single base ratio does not exceed 50%

  G-C content ratio is in the range of 40%- 60%

  Minimize single base repeats of 5 or more

  Most probes are located within 1,200bp of the predicted 3’ end of the

     transcripts

  No >17 continuous matches with all non-target transcripts

  The difference of melting temperature between probe-to-target transcript

     and probe-to-non-target transcripts is >10℃

  Majority of probes show perfect match when blast against genomic

     sequence (i.e. within a single exon)

    Human Whole Genome OneArray^TM Content

          Each Oligonucleotide probe is designed to hybridize to a specific

             target gene described in the most current public domain contents,

             such as Unigene, Cancer Genome Anatomy Project (CGAP),

             BioCarta, Kyoto Encyclopedia of Genes & Genome (KEGG), and

             validated by Human Genome Sequencing

  The most well known and studied target genes will be the first priority

     of probe selection

  Contains 1,082 performance monitoring control probes for hybridization,

     sample quality, and labeling reactions

     For detailed probe annotation of Human Whole Genome OneArray^TM,

     please refer to Phalanx OneArray^TM Support Center:  

     http://support.phalanxbiotech.com/supportTest/index.html