產品資訊 > 模式植物 miRNA 晶片 Model Plant miRNA OneArray® v3

 
  human microarray for gene expression   模式植物 miRNA 晶片 v3 是參考 Sanger miRBase 資料庫 release 17 的序列進行探針設計, 並以自有的高速佈放技術進行晶片生產。v3 版中涵蓋阿拉伯芥、水稻、大豆等七種常見模式植物的 miRNA 探針序列, 於 20119 月上市。
  
 
 
 

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  基 因 探 針

模式植物 miRNA 晶片 v3 是參考 Sanger miRBase 資料庫 release 17 的序列進行探針設計, 共計 1,018 個序列,可對應到 1,817 個成熟的 miRNA。 在探針設計的過程,依據各序列的特性,進行嚴格 Tm 值的把關及調整。 在每一晶片上,每一個 miRNA 序列具有 3 個重覆的探針。



  控 制 探 針

為確保晶片數據品質,華聯技術團隊經過一連串的測試及驗證, 設計一系列的品質控制探針,以監控完整的晶片實驗步驟,包含矩陣校準標記探針、 陽性控制探針及陰性控制探針。
 
 

使用MPmiOA文獻 ( 1 )

 Journal of Experimental Botany. 2013, 64(1):303-15. doi: 10.1093/jxb/ers333.
 Identification and profiling of arsenic stress-induced microRNAs in Brassica juncea 
 Ashish Kumar Srivastava, S.F. D¡¦Souza, Sudhakar Srivastava, Penna Suprasanna
  Abstract
MicroRNAs (miRNAs) constitute a novel mechanism of gene regulation affecting plant development, growth, and stress response. To study the role of miRNAs in arsenic (As) stress, microarray profiling of miRNAs was performed in Brassica juncea using a custom Phalanx Plant OneArray containing 381 unique miRNA probes representing 618 miRNAs from 22 plant species. miRNA microarray hybridization of roots exposed to As for 1 h and 4 h revealed that a total of 69 miRNAs belonging to 18 plant miRNA families had significantly altered expression. The As-responsive miRNAs also exhibited a time- and organ-dependent change in their expression. Putative target prediction for the miRNAs suggested that they regulate various developmental processes (e.g. miR156, miR169, and miR172), sulphur uptake, transport, and assimilation (miR395, miR838, and miR854), and hormonal biosynthesis and/or function (e.g. miR319, miR167, miR164, and miR159). Notable changes were observed in the level of auxins [indole-3-acetic acid (IAA), indole-3- butyric acid, and naphthalene acetic acid], jasmonates [jasmonic acid (JA) and methyl jasmonate], and abscisic acid. The exogenous supply of JA and IAA improved growth of plants under As stress and altered expression of miR167, miR319, and miR854, suggesting interplay of hormones and miRNAs in the regulation of As response. In conclusion, the present work demonstrates the role of miRNAs and associated mechanisms in the plant¡¦s response towards As stress.