Phalanx Biotech Group了解大量基因组数据可能不易分析。因此我们成立了训练有素的生物资讯团队,与学术和行业领域的客户合作,从基础生物学研究到药物发现等各种应用,协助客户解读数据。无论客户是需要我们从原始数据运行基础分析,还是需要进阶分析来回答具体问题,我们都随时提供帮助。我们使用经过验证的分析流程为客户提供最常见问题的快速解答,或者协助构建客制化分析流程,以满足客户的特定需求。华联的生物资讯团队非常乐意与您合作,将您的NGS或生物晶片数据转化为突破性发现!

次世代定序数据分析

生物资讯团队使用经过验证的NGS分析流程处理Illumina / BGI 数据,输出标准和定制的分析结果。请查看以下内容了解我们可以提供的分析类型。

mRNA Sequencing (Gene Expression Analysis)

  • Data QC & filtering
  • Statistical analysis of differentially expressed genes
  • Gene Ontology and Pathway enrichment analysis
  • Principal components analysis

Small RNA Sequencing

  • Data QC & filtering
  • Analyze the expression pattern of known miRNAs
  • Identify novel miRNAs
  • Statistical analysis of differentially expressed miRNAs
  • Target prediction of novel and known miRNAs
  • Gene Ontology and Pathway enrichment analysis of miRNA target genes
  • Principal components analysis

Transcriptome De Novo Assembly

  • Data QC & filtering
  • Transcripts assembly
  • Known and novel transcript identification
  • Differential gene expression analysis
  • Gene Ontology and Pathway enrichment analysis
  • Principal components analysis
  • Novel lncRNA prediction
  • Differential lncRNA expression analysis
  • Up/down stream lncRNAs identified for specific genes
  • Pre-miRNA prediction

16S Metagenomics

  • Data QC & filtering
  • Number of raw reads, number of reads passing QC, number of Operational Taxonomic Units (OTUs)
  • Species composition and abundance analysis
  • Complexity of a single sample (alpha diversity)
  • Complexity differences between different samples (beta diversity, samples ≥ 2)
  • Principal component analysis (samples ≥ 3)
  • Clustering of species composition among samples (Samples ≥ 3)

ChIP-seq

  • Data QC & filtering
  • Align reads to the reference genome
  • Peak analysis (peak length and depth)
  • Peak annotation (peak distribution, Gene Ontology and Pathway analysis of peak related gene)
  • Identify differential peaks between samples
  • Differential peaks annotation (differential peaks distribution, GO and Pathway analysis of differential peak related gene)
  • Motif analysis

Whole Exome Sequencing

  • Data QC & filtering
  • Align reads to the human reference genome
  • SNP calling and annotation
  • SNP validation and comparison
  • Functional prediction of SNPs
  • InDel calling and annotation
  • InDel validation and comparison

生物晶片数据分析

我们的生物资讯学团队在处理OneArray®、Affymetrix、Agilent,Illumina和其他平台的数据分析方面拥有丰富的经验。请查看以下内容了解我们可以提供的分析类型。

OneArray®

Our basic OneArray®data analysis package includes normalization, identification of differentially expressed genes, principal components analysis, hierarchical clustering, and Gene Ontology and Pathway enrichment analysis.

Agilent

Our basic Agilent data analysis package includes normalization, identification of differentially expressed genes, principal components analysis, and hierarchical clustering.

Affymetrix

Our basic Affymetrix data analysis package includes normalization, identification of differentially expressed genes, principal components analysis, and hierarchical clustering.

Illumina

Our basic Illumina data analysis package includes normalization, identification of differentially expressed genes, principal components analysis, and hierarchical clustering.

Advanced Analysis

  • Gene Ontology and Pathway enrichment analyses: using a set of differentially expressed genes, an enrichment analysis will find which GO terms and pathways are over-represented (or under-represented) using annotations for that gene set.
  • CMAP: a useful tool in the drug discovery process, OneArray® microarray results can be input into our CMAP pipeline in order to identify/predict the effects of different drugs on different compounds without having to run the wet lab experiments. Protein target prediction and advanced pathway analysis can be performed as subsequent analyses in order to give an even more complete picture of the experiment.

生物晶片或次世代定序进阶分析

我们的生物资讯团队在生物晶片和次世代定序进阶分析拥有丰富的经验。请查看以下内容了解我们可以提供的分析类型。

Functional Analyses

Target gene prediction service, integrated analysis of mRNA and microRNA expression

Pattern Recognition

Heat map, unsupervised clustering analysis, functional clustering analysis, classification analysis

Visualization Analyses

Bar plot, box plot, volcano plot, Venn diagram, pie chart, principal components analysis 2D/3D plot, biological network data integration/analysis/visualization services

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