華聯生物科技提供大量微阵列平台,以满足您的表达谱分析需求。我们的专业实验室技术人员拥有70多年的综合经验,严格的质量控制流程将确保您获得高质量的表达数据。

OneArray® 基因表达分析服务

Phalanx Biotech Group的生物晶片服务实验室使用我们专有的OneArray®平台进行基因表达分析服务。基于原厂自行研发及制造的优势,此平台已经过不断的验证,测试和更新,因此我们可以以实惠的价格为您带来最佳的基因表达解读。此外,由于拥有多年执行服务经验,我们的专家也会协助您进行实验设计、检体运送、检体品质分析及后续实验分析。

特色

  • OneArray® gene expression profiling is available for the following species: Human (HOA), Mouse (MOA), Rat (ROA), Rice (RiOA), and Yeast (YOA).
  • 60-mer probes were engineered using specific lengths to match melting temperatures for superior hybridization performance.
  • Microarray service provider for 10+ years.
  • Outstanding technical support from microarray experts.
  • Professional lab technicians with 70+ years of combined experience.
  • Our complete microarray service workflow includes RNA QC, amplification, labeling, hybridization, scanning, data extraction, and data analysis.

总览

Species available:
SpeciesOneArray PlatformNo. of Genome Probes
HumanHOA v728,264
MouseMOA v226,423
RatROA v220,715
RiceRiOA v121,179
YeastYOA v16,958
Workflow:
StepMethodQC metrics
RNA isolation (optional)Trizol/RNeasy/etc.Need > 3ug
RNA QCNanoDrop260/230 > 1.5, 260/280 > 1.8
 Agilent BioanalyzerRIN > 6
RNA amplificationEberwine Method with aa-dUTP incorporationInternally validated thresholds for aRNA yield measured with NanoDrop
RNA labelingCy-dye coupling to aa-dUTPInternally validated thresholds for labeling efficiency measured with NanoDrop
HybridizationPhalanx Method 
Data extractionAgilent Extended Dynamic RangeInternally validated metrics for hybridization efficiency using control microarray probes
Data Analysis:
  • Data normalization
  • Identify differentially expressed genes (unlimited statistical comparisons)
  • Principal components analysis
  • Hierarchical clustering
  • Gene Ontology and Pathway enrichment analyses (for each list of differentially expressed genes)

资源

OneArray® miRNA表达分析服务

Phalanx Biotech Group的生物晶片服务实验室使用我们专有的OneArray®平台进行miRNA表达分析服务。基于原厂自行研发及制造的优势,此平台已经过不断的验证,测试和更新,因此我们可以以实惠的价格为您带来最佳的miRNA表达解读。再者,由于拥有多年执行服务经验,我们的专家也会协助您进行实验设计、检体运送、检体品质分析及后续实验分析。

特色

  • OneArray® miRNA expression profiling is available for the following species: Human (HmiOA v7), Mouse & Rat (MRmiOA v7), and Model Plant species (MPmiOA v3)
  • Probes contain miRNA-specific sequences and a proprietary spacer designed to enhance the hybridization sensitivity to miRNAs.
  • Microarray service provider for 10+ years.
  • Outstanding technical support from microarray experts.
  • Professional lab technicians with 70+ years of combined experience.
  • Our complete microarray service workflow includes RNA QC, miRNA enrichment, labeling, hybridization, scanning, data extraction, and data analysis.

总览

Species available:
SpeciesFormatAgilent Design IDNo. of Genome Probes
Human8 x 60K07236326,083 Genes 30,606 lncRNAs
Mouse8 x 60K07480927,122 Genes 4,578 lncRNAs
Rat8 x 60K07403630,584
Arabidopsis4 x 44K02116943,803
Barley4 x 44K02162343,803
Bovine4 x 44K02364743,803
Brassica4 x 44K02252043,803
C. elegans4 x 44K02018643,803
Canine4 x 44K02119343,803
Chicken4 x 44K02644143,803
Cotton4 x 44K02252343,803
Drosophila4 x 44K02179143,803
E. coli8 x 15K02009715,208
Horse4 x 44K02132243,803
Magnaporthe4 x 44K01506015,170 Magnaporthe 6,325 Rice
Mosquito4 x 44K02044943,803
Porcine4 x 44K02644043,803
Rabbit4 x 44K02090843,803
Rhesus4 x 44K02680643,803
Rice4 x 44K01524143,803
Salmon4 x 44K02093843,803
Sheep8 x 15K01992115,208
Tobacco4 x 44K02111343,803
Tomato4 x 44K02227043,803
Wheat4 x 44K02229743,803
Xenopus4 x 44K02344843,803
Yeast8 x 15K0163226,256
Zebrafish4 x 44K02643743,803
Workflow:
StepMethodQC metrics
RNA isolation (optional)Trizol/RNeasy/etc.Need > 0.5ug
RNA QCNanoDrop260/230 > 0.7, 260/280 > 1.8
 Agilent BioanalyzerRIN > 7
RNA amplification and labelingAgilent Low Input Quick Amp Labeling KitInternally validated thresholds for aRNA yield and labeling efficiency measured with NanoDrop
HybridizationAgilent ProtocolSee below
Data extractionAgilent Extended Dynamic RangeInternally validated metrics for hybridization efficiency using control microarray probes
Data Analysis:
  • Data normalization
  • Identify differentially expressed genes (unlimited statistical comparisons)
  • Principal components analysis
  • Hierarchical clustering

资源

安捷伦生物晶片服务

Phalanx Biotech Group除了本身的产品之外,也提供相关行业领先者–安捷伦生物晶片服务,内容包含28种模式生物。由于拥有多年执行服务经验,我们的专家会协助您进行实验设计、检体运送、检体品质分析及后续实验分析。

特色

  • OneArray® gene expression profiling is available for the following species: Human (HOA), Mouse (MOA), Rat (ROA), Rice (RiOA), and Yeast (YOA).
  • 60-mer probes were engineered using specific lengths to match melting temperatures for superior hybridization performance.
  • Microarray service provider for 10+ years.
  • Outstanding technical support from microarray experts.
  • Professional lab technicians with 70+ years of combined experience.
  • Our complete microarray service workflow includes RNA QC, amplification, labeling, hybridization, scanning, data extraction, and data analysis.

总览

Species available:
SpeciesFormatAgilent Design IDNo. of Genome Probes
Human8 x 60K07236326,083 Genes 30,606 lncRNAs
Mouse8 x 60K07480927,122 Genes 4,578 lncRNAs
Rat8 x 60K07403630,584
Arabidopsis4 x 44K02116943,803
Barley4 x 44K02162343,803
Bovine4 x 44K02364743,803
Brassica4 x 44K02252043,803
C. elegans4 x 44K02018643,803
Canine4 x 44K02119343,803
Chicken4 x 44K02644143,803
Cotton4 x 44K02252343,803
Drosophila4 x 44K02179143,803
E. coli8 x 15K02009715,208
Horse4 x 44K02132243,803
Magnaporthe4 x 44K01506015,170 Magnaporthe 6,325 Rice
Mosquito4 x 44K02044943,803
Porcine4 x 44K02644043,803
Rabbit4 x 44K02090843,803
Rhesus4 x 44K02680643,803
Rice4 x 44K01524143,803
Salmon4 x 44K02093843,803
Sheep8 x 15K01992115,208
Tobacco4 x 44K02111343,803
Tomato4 x 44K02227043,803
Wheat4 x 44K02229743,803
Xenopus4 x 44K02344843,803
Yeast8 x 15K0163226,256
Zebrafish4 x 44K02643743,803
Workflow:
StepMethodQC metrics
RNA isolation (optional)Trizol/RNeasy/etc.Need > 0.5ug
RNA QCNanoDrop260/230 > 0.7, 260/280 > 1.8
 Agilent BioanalyzerRIN > 7
RNA amplification and labelingAgilent Low Input Quick Amp Labeling KitInternally validated thresholds for aRNA yield and labeling efficiency measured with NanoDrop
HybridizationAgilent ProtocolSee below
Data extractionAgilent Extended Dynamic RangeInternally validated metrics for hybridization efficiency using control microarray probes
Data Analysis:
  • Data normalization
  • Identify differentially expressed genes (unlimited statistical comparisons)
  • Principal components analysis
  • Hierarchical clustering

资源

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