次世代定序(NGS)是观察您所研究物种之遗传图谱的有力工具。透过不同的表现型(Phenotype), 您可以寻找相关SNP、变异或致病位点;进一步了解药物作用如何调控整个基因组编码区的表达;亦或是发现新的转录序列;甚至探索全新的生物遗传组成。

Phalanx Biotech Group使用Illumina / BGI平台提供NGS服务,协助您取得一系列的生物实验数据,包含基因组学(Genomics)、转录组学(Transcriptomics)、表观遗传学(Epigenomics)和宏观基因组学(Metagenomics)等。我们可以依照您的需求,提供您实验设计、检体准备、定序、分析…等的各种协助。

NGS服务

RNA-Seq

RNA-Seq Service

RNA sequencing is a type of next-generation sequencing used to target regions of the genome that are transcribed into
messenger RNA (mRNA) and may subsequently translate into protein. RNA-seq is used to identify and quantify
transcriptional regulation of the genome.

Protocols specific to the library preparation stage are used to isolate mRNA and rid samples of rRNA, which comprises
approximately 80% of the RNA pool.

Typical sequencing configurations:
ConfigurationmRNA-seq
Library Preparation ProtocolStrand-specific, polyA Selection, paired-end
Recommended Throughput20-40 million reads per sample
PlatformIllumina HiSeq

*Sequencing configuration is project specific.

Sample submission requirements for RNA-Sequencing:
SAMPLE TYPEAMOUNTRINCONCENTRATION
Total RNA in Rnase-free water, on dry ice2ug (min > 500ng)6.050 ng/uL
RNA-Seq Applications:

RNA-seq may be used for gene expression profiling, identification of novel transcripts, identification of alternative splice events and transcriptome assembly.

Gene expression profiling by way of RNA-seq is utilized in drug development experiments, cancer research, gut microbiome studies and many more. Comparative analysis of transcript abundance between experimental groups provides unique insight into the interplay between gene and environment.

Small RNA-Seq

Small RNA-Seq Service

Small RNA sequencing is used to target small RNA molecules including: miRNA, ncRNA, snRNA, etc.

Common sequencing configurations:
Configurationsmall RNA-seq
Library Preparation ProtocolCustomizable size selection using small RNA library prep kits
Recommended Throughput7-10 million reads per sample
PlatformIllumina NextSeq
Sample submission requirements for Small RNA-Sequencing:
Sample TypeMinimum AmountRINMinimum Concentration
Total RNA200 ng>7.020 g/uL

ChIP-Seq

ChIP-Seq Sample submission requirements:
Sample TypeMinimum AmountMinimum Concentration
DNA5 ng1 ng/uL
RNA QC Requirements:
  • A260/280 > 2
  • A260/230 = 2.0~2.2
Recommended Sequencing Specifications:
  • Single end 1x50 bp
  • 20M, 40M, 75M, or 100M reads

16s Metagenomics

Smple submission requirements:
Sample TypeMinimum AmountMinimum Concentration
DNA12.5 ng1.5 ng/uL
RNA QC Requirements:
  • A260/280 > 2
  • A260/230 = 2.0~2.2
Recommended Sequencing Specifications:
  • Paired end 2x300bp
  • 100K coverage

Whole Exome Sequencing (WES)

Sample submission requirements:
Sample TypeMinimum AmountMinimum Concentration
DNA12.5 ng1.5 ng/uL
RNA QC Requirements:
  • A260/280 > 2
  • 260/230 = 2.0~2.2
Recommended Sequencing Specifications:
  • aired end 2x300 bp
  • 100K coverage

特色

  • 完整的工作流程:我们可以依照您的需求,执行样品核酸提取、建库(library construction)、定序、基础数据分析和进阶数据分析。
  • 低产量样品:如果您的样品类型为有限的RNA / DNA,请联系我们以了解我们能提供的特殊专属流程。
  • 种类繁多:我们处理过各样基因组(人类,小鼠,大鼠,仓鼠,细菌等)物种的样本。
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