次世代定序(NGS)是观察您所研究物种之遗传图谱的有力工具。透过不同的表现型(Phenotype), 您可以寻找相关SNP、变异或致病位点;进一步了解药物作用如何调控整个基因组编码区的表达;亦或是发现新的转录序列;甚至探索全新的生物遗传组成。
Phalanx Biotech Group使用Illumina / BGI平台提供NGS服务,协助您取得一系列的生物实验数据,包含基因组学(Genomics)、转录组学(Transcriptomics)、表观遗传学(Epigenomics)和宏观基因组学(Metagenomics)等。我们可以依照您的需求,提供您实验设计、检体准备、定序、分析…等的各种协助。
NGS服务
RNA-Seq
RNA-Seq Service
RNA sequencing is a type of next-generation sequencing used to target regions of the genome that are transcribed into
messenger RNA (mRNA) and may subsequently translate into protein. RNA-seq is used to identify and quantify
transcriptional regulation of the genome.
Protocols specific to the library preparation stage are used to isolate mRNA and rid samples of rRNA, which comprises
approximately 80% of the RNA pool.
Typical sequencing configurations:
Configuration | mRNA-seq |
---|---|
Library Preparation Protocol | Strand-specific, polyA Selection, paired-end |
Recommended Throughput | 20-40 million reads per sample |
Platform | Illumina HiSeq |
*Sequencing configuration is project specific.
Sample submission requirements for RNA-Sequencing:
SAMPLE TYPE | AMOUNT | RIN | CONCENTRATION |
---|---|---|---|
Total RNA in Rnase-free water, on dry ice | 2ug (min > 500ng) | 6.0 | 50 ng/uL |
RNA-Seq Applications:
RNA-seq may be used for gene expression profiling, identification of novel transcripts, identification of alternative splice events and transcriptome assembly.
Gene expression profiling by way of RNA-seq is utilized in drug development experiments, cancer research, gut microbiome studies and many more. Comparative analysis of transcript abundance between experimental groups provides unique insight into the interplay between gene and environment.
Small RNA-Seq
Small RNA-Seq Service
Small RNA sequencing is used to target small RNA molecules including: miRNA, ncRNA, snRNA, etc.
Common sequencing configurations:
Configuration | small RNA-seq |
---|---|
Library Preparation Protocol | Customizable size selection using small RNA library prep kits |
Recommended Throughput | 7-10 million reads per sample |
Platform | Illumina NextSeq |
Sample submission requirements for Small RNA-Sequencing:
Sample Type | Minimum Amount | RIN | Minimum Concentration |
---|---|---|---|
Total RNA | 200 ng | >7.0 | 20 g/uL |
ChIP-Seq
ChIP-Seq Sample submission requirements:
Sample Type | Minimum Amount | Minimum Concentration |
---|---|---|
DNA | 5 ng | 1 ng/uL |
RNA QC Requirements:
- A260/280 > 2
- A260/230 = 2.0~2.2
Recommended Sequencing Specifications:
- Single end 1x50 bp
- 20M, 40M, 75M, or 100M reads
16s Metagenomics
Smple submission requirements:
Sample Type | Minimum Amount | Minimum Concentration |
---|---|---|
DNA | 12.5 ng | 1.5 ng/uL |
RNA QC Requirements:
- A260/280 > 2
- A260/230 = 2.0~2.2
Recommended Sequencing Specifications:
- Paired end 2x300bp
- 100K coverage
Whole Exome Sequencing (WES)
Sample submission requirements:
Sample Type | Minimum Amount | Minimum Concentration |
---|---|---|
DNA | 12.5 ng | 1.5 ng/uL |
RNA QC Requirements:
- A260/280 > 2
- 260/230 = 2.0~2.2
Recommended Sequencing Specifications:
- aired end 2x300 bp
- 100K coverage
特色
- 完整的工作流程:我们可以依照您的需求,执行样品核酸提取、建库(library construction)、定序、基础数据分析和进阶数据分析。
- 低产量样品:如果您的样品类型为有限的RNA / DNA,请联系我们以了解我们能提供的特殊专属流程。
- 种类繁多:我们处理过各样基因组(人类,小鼠,大鼠,仓鼠,细菌等)物种的样本。