生物晶片服務綜覽
MicroArray Services Overview
華聯生物科技專注於基因檢測平台的開發，具備基因微陣列晶片、次世代定序、大數據分析等基因檢測分析技術，可滿足您表達譜分析的多樣需求。我們擁有專業認證實驗室、專業技術人員、高規基因檢測設備，為您提供最完整的科學研究與基因檢測服務。
OneArray® 基因表達分析服務
Phalanx Biotech Group的生物晶片服務實驗室使用我們專有的OneArray®平台進行基因表達分析服務。基於原廠自行研發及製造的優勢，此平台已經過不斷的驗證，測試和更新，因此我們可以以實惠的價格為您帶來最佳的基因表達解讀。此外，由於擁有多年執行服務經驗，我們的專家也會協助您進行實驗設計、檢體運送、檢體品質分析及後續實驗分析。
特色
- OneArray® gene expression profiling is available for the following species: Human (HOA), Mouse (MOA), Rat (ROA), Rice (RiOA), and Yeast (YOA).
- 60-mer probes were engineered using specific lengths to match melting temperatures for superior hybridization performance.
- Microarray service provider for 10+ years.
- Outstanding technical support from microarray experts.
- Professional lab technicians with 70+ years of combined experience.
- Our complete microarray service workflow includes RNA QC, amplification, labeling, hybridization, scanning, data extraction, and data analysis.
總覽
Species available:
| Species | OneArray Platform | No. of Genome Probes |
|---|---|---|
| Human | HOA v7 | 28,264 |
| Mouse | MOA v2 | 26,423 |
| Rat | ROA v2 | 20,715 |
| Rice | RiOA v1 | 21,179 |
| Yeast | YOA v1 | 6,958 |
Workflow:
| Step | Method | QC metrics |
|---|---|---|
| RNA isolation (optional) | Trizol/RNeasy/etc. | Need > 3ug |
| RNA QC | NanoDrop | 260/230 > 1.5, 260/280 > 1.8 |
| Agilent Bioanalyzer | RIN > 6 | |
| RNA amplification | Eberwine Method with aa-dUTP incorporation | Internally validated thresholds for aRNA yield measured with NanoDrop |
| RNA labeling | Cy-dye coupling to aa-dUTP | Internally validated thresholds for labeling efficiency measured with NanoDrop |
| Hybridization | Phalanx Method | |
| Data extraction | Agilent Extended Dynamic Range | Internally validated metrics for hybridization efficiency using control microarray probes |
Data Analysis:
- Data normalization
- Identify differentially expressed genes (unlimited statistical comparisons)
- Principal components analysis
- Hierarchical clustering
- Gene Ontology and Pathway enrichment analyses (for each list of differentially expressed genes)
資源
BROCHURES
SAMPLE REPORTS
SAMPLE SUBMISSION
TECH NOTES
OneArray® miRNA表達分析服務
Phalanx Biotech Group的生物晶片服務實驗室使用我們專有的OneArray®平台進行miRNA表達分析服務。基於原廠自行研發及製造的優勢，此平台已經過不斷的驗證，測試和更新，因此我們可以以實惠的價格為您帶來最佳的miRNA表達解讀。再者，由於擁有多年執行服務經驗，我們的專家也會協助您進行實驗設計、檢體運送、檢體品質分析及後續實驗分析。
特色
- OneArray® miRNA expression profiling is available for the following species: Human (HmiOA v7), Mouse & Rat (MRmiOA v7), and Model Plant species (MPmiOA v3)
- Probes contain miRNA-specific sequences and a proprietary spacer designed to enhance the hybridization sensitivity to miRNAs.
- Microarray service provider for 10+ years.
- Outstanding technical support from microarray experts.
- Professional lab technicians with 70+ years of combined experience.
- Our complete microarray service workflow includes RNA QC, miRNA enrichment, labeling, hybridization, scanning, data extraction, and data analysis.
總覽
Species available:
| Species | Format | Agilent Design ID | No. of Genome Probes |
|---|---|---|---|
| Human | 8 x 60K | 072363 | 26,083 Genes 30,606 lncRNAs |
| Mouse | 8 x 60K | 074809 | 27,122 Genes 4,578 lncRNAs |
| Rat | 8 x 60K | 074036 | 30,584 |
| Arabidopsis | 4 x 44K | 021169 | 43,803 |
| Barley | 4 x 44K | 021623 | 43,803 |
| Bovine | 4 x 44K | 023647 | 43,803 |
| Brassica | 4 x 44K | 022520 | 43,803 |
| C. elegans | 4 x 44K | 020186 | 43,803 |
| Canine | 4 x 44K | 021193 | 43,803 |
| Chicken | 4 x 44K | 026441 | 43,803 |
| Cotton | 4 x 44K | 022523 | 43,803 |
| Drosophila | 4 x 44K | 021791 | 43,803 |
| E. coli | 8 x 15K | 020097 | 15,208 |
| Horse | 4 x 44K | 021322 | 43,803 |
| Magnaporthe | 4 x 44K | 015060 | 15,170 Magnaporthe 6,325 Rice |
| Mosquito | 4 x 44K | 020449 | 43,803 |
| Porcine | 4 x 44K | 026440 | 43,803 |
| Rabbit | 4 x 44K | 020908 | 43,803 |
| Rhesus | 4 x 44K | 026806 | 43,803 |
| Rice | 4 x 44K | 015241 | 43,803 |
| Salmon | 4 x 44K | 020938 | 43,803 |
| Sheep | 8 x 15K | 019921 | 15,208 |
| Tobacco | 4 x 44K | 021113 | 43,803 |
| Tomato | 4 x 44K | 022270 | 43,803 |
| Wheat | 4 x 44K | 022297 | 43,803 |
| Xenopus | 4 x 44K | 023448 | 43,803 |
| Yeast | 8 x 15K | 016322 | 6,256 |
| Zebrafish | 4 x 44K | 026437 | 43,803 |
Workflow:
| Step | Method | QC metrics |
|---|---|---|
| RNA isolation (optional) | Trizol/RNeasy/etc. | Need > 0.5ug |
| RNA QC | NanoDrop | 260/230 > 0.7, 260/280 > 1.8 |
| Agilent Bioanalyzer | RIN > 7 | |
| RNA amplification and labeling | Agilent Low Input Quick Amp Labeling Kit | Internally validated thresholds for aRNA yield and labeling efficiency measured with NanoDrop |
| Hybridization | Agilent Protocol | See below |
| Data extraction | Agilent Extended Dynamic Range | Internally validated metrics for hybridization efficiency using control microarray probes |
Data Analysis:
- Data normalization
- Identify differentially expressed genes (unlimited statistical comparisons)
- Principal components analysis
- Hierarchical clustering
資源
BROCHURES
SAMPLE REPORTS
SAMPLE SUBMISSION
TECH NOTES
安捷倫生物晶片服務
Phalanx Biotech Group除了本身的產品之外，也提供相關行業領先者–安捷倫生物晶片服務，內容包含28種模式生物。由於擁有多年執行服務經驗，我們的專家會協助您進行實驗設計、檢體運送、檢體品質分析及後續實驗分析。
特色
- OneArray® gene expression profiling is available for the following species: Human (HOA), Mouse (MOA), Rat (ROA), Rice (RiOA), and Yeast (YOA).
- 60-mer probes were engineered using specific lengths to match melting temperatures for superior hybridization performance.
- Microarray service provider for 10+ years.
- Outstanding technical support from microarray experts.
- Professional lab technicians with 70+ years of combined experience.
- Our complete microarray service workflow includes RNA QC, amplification, labeling, hybridization, scanning, data extraction, and data analysis.
總覽
Species available:
| Species | Format | Agilent Design ID | No. of Genome Probes |
|---|---|---|---|
| Human | 8 x 60K | 072363 | 26,083 Genes 30,606 lncRNAs |
| Mouse | 8 x 60K | 074809 | 27,122 Genes 4,578 lncRNAs |
| Rat | 8 x 60K | 074036 | 30,584 |
| Arabidopsis | 4 x 44K | 021169 | 43,803 |
| Barley | 4 x 44K | 021623 | 43,803 |
| Bovine | 4 x 44K | 023647 | 43,803 |
| Brassica | 4 x 44K | 022520 | 43,803 |
| C. elegans | 4 x 44K | 020186 | 43,803 |
| Canine | 4 x 44K | 021193 | 43,803 |
| Chicken | 4 x 44K | 026441 | 43,803 |
| Cotton | 4 x 44K | 022523 | 43,803 |
| Drosophila | 4 x 44K | 021791 | 43,803 |
| E. coli | 8 x 15K | 020097 | 15,208 |
| Horse | 4 x 44K | 021322 | 43,803 |
| Magnaporthe | 4 x 44K | 015060 | 15,170 Magnaporthe 6,325 Rice |
| Mosquito | 4 x 44K | 020449 | 43,803 |
| Porcine | 4 x 44K | 026440 | 43,803 |
| Rabbit | 4 x 44K | 020908 | 43,803 |
| Rhesus | 4 x 44K | 026806 | 43,803 |
| Rice | 4 x 44K | 015241 | 43,803 |
| Salmon | 4 x 44K | 020938 | 43,803 |
| Sheep | 8 x 15K | 019921 | 15,208 |
| Tobacco | 4 x 44K | 021113 | 43,803 |
| Tomato | 4 x 44K | 022270 | 43,803 |
| Wheat | 4 x 44K | 022297 | 43,803 |
| Xenopus | 4 x 44K | 023448 | 43,803 |
| Yeast | 8 x 15K | 016322 | 6,256 |
| Zebrafish | 4 x 44K | 026437 | 43,803 |
Workflow:
| Step | Method | QC metrics |
|---|---|---|
| RNA isolation (optional) | Trizol/RNeasy/etc. | Need > 0.5ug |
| RNA QC | NanoDrop | 260/230 > 0.7, 260/280 > 1.8 |
| Agilent Bioanalyzer | RIN > 7 | |
| RNA amplification and labeling | Agilent Low Input Quick Amp Labeling Kit | Internally validated thresholds for aRNA yield and labeling efficiency measured with NanoDrop |
| Hybridization | Agilent Protocol | See below |
| Data extraction | Agilent Extended Dynamic Range | Internally validated metrics for hybridization efficiency using control microarray probes |
Data Analysis:
- Data normalization
- Identify differentially expressed genes (unlimited statistical comparisons)
- Principal components analysis
- Hierarchical clustering
資源
BROCHURES
SAMPLE REPORTS
SAMPLE SUBMISSION
TECH NOTES
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